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MB? 488 DBCO is a bright, green fluorescent probe with excitation ideally suited for the 488 nm laser line. MB? 488 is bright, exceedingly photostable and pH-insensitive fluorescent dye that can be used in place of Fluorescein, Alexa Fluor 488®, DyLight 488® and other dyes excitable at a wavelength of 488 nm.

MB? 488 DBCO dye reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern MB? 488 DBCO is an ideal alternative to copper requiring fluorescent alkynes.

MB? 488 DBCO reagent is not suitable for staining intracellular components of fixed and permeabilized cells due to high backgrounds.